Evaluation of Antioxidant Activity of Cedrela toona Roxb. Leaf Extracts
Abstract
Antioxidant potency of fruits of Cedrela toona Roxb. crude methanol and its fractionated extracts (hexane, acetone and water) have been investigated, employing three different established in vitro testing systems, such as scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power assay and β-carotene method. The methanol extract of fruits of Cedrela toona Roxb. showed the best DPPH scavenging activity with the lowest EC 50 34 μg/ml, followed by the acetone (EC 50 39 μg/ml), hexane (EC 50 48 μg/ml) and water extract (EC 50 54 μg/ml). The reducing power of Cedrela toona Roxb. fruit extracts increased steadily with increasing concentrations and varied significantly with different concentrations. The methanol and acetone extracts appeared to possess the highest significant reducing activity among the extracts. The stronger reducing power in the methanol and acetone extracts was probably due to the concentration of antioxidant compounds like flavonoids and phenolics in the extract. In the β-carotene bleaching assay, the antioxidant activities of all the fruits extracts gradually increased with increasing concentration of the extracts and varied significantly with different concentrations. The water extract showed the lowest significant antioxidant activity, while the methanol extract showed the highest significant antioxidant activity. The high antioxidant activity of methanol extract tested using β-carotene model may be correlated with the high phenolic content of the methanol extract. In conclusion, antioxidant study suggested that fruits of Cedrela toona Roxb. are potential source of natural antioxidants. However, further investigations on in vivo antioxidant activities are highly recommended.
In the present study, the antioxidant potency of Cedrela toona Roxb. crude methanol and its fractionated extracts (hexane, acetone and water) have been investigated, employing three different established in vitro testing systems, such as scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power assay and β-carotene method. There is no antioxidant study reported for Cedrela toona Roxb.Thus, antioxidant activity of Cedrela toona Roxb. was evaluated as it had not been determined previously.
References
2. Loupee D, Oteng- Amoaka A.A, Brink M. Timber 1. Vol1, vol7, PROTA publishers, 2008; 557-559.
3. http://en.wikipedia.org/wiki/Toon.
4. Kashyapa K, Chand R. The useful plants of India. National Institute of Sciences Communication and Information Resources, New Delhi, 2006; 112-113.
5. Nadkarni A K. Indian Materia Medica. Edn 3, Vol I, Popular prakashan, 2009; 1908.
6. Pullaiah, T. Biodiversity in India. Vol 4, Published by Regency Publication, 2006; 160.
7. Pandya N, Santani D, Jain S, Antioxidant activity of ezetimibe in hypercholesterolemic rats, Indian J Pharmacol, 2006; 38(3):205, 206.
8. Sim KS, Sri Nurestri AM, Norhanom AW. Phenolic content and antioxidant activity of Pereskia grandifolia Haw. (Cactaceae) extracts. Phcog Mag 2010; 6:248-54.
9. Tanmayee Mishra, Arvind K Goyal, Sushil K Middha, Arnab Sen, “Antioxidative properties of Canna edulis Ker-Gawl.”, Ind J Nat Prod Resour 2011; 2(3):315-321.
10. Koleva I. I., Van Beek T. A., Linssen J.P.H., De Groot A, Evstatieva L.N. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis. 2002; 13:8-17.
11. Kumaran A. and Karunakaran R. J.; In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India, Food Science and Technology, 2007; 40(2):344-352.
12. Gupta M., Mazumdar U. K., Gomathi P., Kumar R. S.; Antioxidant and Free Radical Scavenging Activities of Ervatamia coronaria Stapf. Leaves, Iranian Journal of Pharmaceutical Research, 2004; 2:119-126.
The International Journal of Innovative Science & Technology affairs require a formal written transfer of copyright from the author(s) for each article published. We therefore ask you to complete and return this form, retaining a copy for your records. Your cooperation is essential and appreciated. Any delay will result in a delay in publication.
I/we have read and agree with the terms and conditions stated Page 2 of this agreement and I/we hereby confirm the transfer of all copyrights in and relating to the above-named manuscript, in all forms and media, now or hereafter known, to the International Journal of Drug Regulatory affairs, effective from the date stated below. I/we acknowledge that the IJDRA is relying on this agreement in publishing the above-named manuscript. However, this agreement will be null and void if the manuscript is not published in the IJIST.
Download link for COPYRIGHT FORM
