Himalayan Journal of Health Sciences

Structure-Based Virtual Screening of Small Molecules Targeting Key Proteins of Neisseria gonorrhoeae

2025-06-25T16:31:30-04:00

Objectives: To identify potential small molecule inhibitors against key resistance-associated proteins of Neisseria gonorrhoeae using a molecular docking-based virtual screening approach, addressing the urgent need for novel therapeutics in light of rising drug resistance.

Materials and Methods A computational study employing virtual screening of phytochemicals and standard antibiotics against selected N. gonorrhoeae proteins using SwissDock and CB-Dock, followed by interaction analysis through Discovery Studio Visualizer.

Interventions. Docking of 30 structurally diverse phytochemicals and four standard antibiotics against four essential proteins of N. gonorrhoeae: Penicillin-binding Protein 2 (PBP2; PDB ID: 3equ), Hypothetical Protein with bound ppGpp (PDB ID: 5vog), Adhesin Complex Protein (PDB ID: 6gq4)

Main Outcome Measures: Evaluation of binding affinities, docking scores, and molecular interaction patterns to determine the most promising small molecule inhibitors.

Results: Several phytochemicals exhibited strong binding affinities and favorable interactions with the target proteins, in some cases outperforming standard antibiotics. Detailed interaction profiles revealed key residues involved in ligand binding and supported the potential of selected compounds as inhibitors.

Conclusion: The in silico findings highlight promising phytochemical candidates for further experimental validation. These results contribute to the development of novel therapeutic strategies to combat extensively drug-resistant N. gonorrhoeae strains and support the role of natural compounds in antimicrobial drug discovery.

A Review on Tarka and Nyaya in Ayurveda: Role of Logical Reasoning in Diagnosis and Treatment

2025-06-25T16:55:20-04:00

Tarka or reasoning or rational inquiry in Sanskrit, is a basic philosophical concept within Indian philosophical schools, especially the Nyaya school. It involves formalized argument, profound introspection and sophisticated analysis, making it an invaluable tool for discerning truth from falsity. Tarka Shastra, the reasoning science, is a methodical structure for dialectics and argumentation that includes important techniques like Purva paksha and Apara paksha. Closely associated with Nyaya philosophy, Tarka is used as a vital epistemological instrument to evaluate sources of knowledge, solve philosophical conflicts, and guide the intellect towards sound conclusions. Nyaya, although a separate but ancillary system, provides a detailed framework of sixteen categories for ordering reasoning and acquisition of knowledge. This essay discusses the complex function of Tarka, its use in different schools of philosophy, and its inclusion within Nyaya to aid the final philosophical objective Moksha through proper knowledge.

Ayurvedic Concept, Significance and Therapeutic Value of Pishti Kalpas

2025-06-25T17:08:45-04:00

A vast range of preparations, including Kharaliya Rasayana and Kupipakwa Rasayana, are included in the field of Rasa Shastra, the traditional Ayurvedic branch devoted to the creation of herbo-mineral remedies. The type of raw materials utilized and the particular steps are involved in the formulations of specific Kalpas of Rasa Shastra. Pishti is one such Kalpa of this branch which holds a prominent place in Rasa Shastra. Pishti is a unique Ayurvedic concoction that is especially useful in treating many illnesses. Pishtis are prepared by repeated controlled triturating with herbal solutions. This procedure guarantees that raw materials are transformed into a very fine, bio-available state. Pravala Pishti and Mukta Pishti are important formulations amongst this category. The production of Pishti, its historical development and its clinical significance in Ayurvedic medicine are discussed in this article.

Trisutra Concepts of Ayurveda and its Significance in Disease Diagnosis and Treatment

2025-08-15T18:16:36-04:00

The idea of Trisutra, which includes Hetu, Linga and Aushadha, is described by Ayurveda with the goal of promoting the health of healthy people and the recovery of patients from a variety of illnesses. The three aphorisms known as Trisutra; Hetu, Linga and Oushadha encapsulate this age-old science. Hetu relates to the causative variables that control the preservation of health as well as the causes of various diseases. Hetu resembles the primary causes of sickness; Linga explained the clinical signs of both healthy and sick situations, while Oushadha mentioned medications, including those that aid in returning the body to its normal state. Oushadha is used to treat a sick patient as well as to maintain and improve the health of a healthy one. Oushadha also discusses the application of healthy foods and a disciplined way of living to treat illnesses. This article explains Trisutra concepts of Ayurveda and its significance in disease diagnosis and treatment.